Structural confirmation of B1-doped PVA/collagen-fabricated composite films was carried out using FTIR spectroscopic analysis (between 4000 and 500 cm –1) by using a Shimadzu FTIR instrument (Model: IR Prestige-21, Shimadzu Corporation, Japan). The films were cooled to room temperature, slowly peeled off from the Petri plate and stored in a polyethylene bag. Different concentrations of B1 (0.25, 0.5, 1, 2, and 4%) (w/v) was dissolved in dimethyl sulfoxide were added to PVA (2%)/collagen (2%) blend solution and it was stirred at 4 ☌ (350 rpm) for 12 h on a magnetic stirrer, further sonicated for 5 min, and it was uniformly poured onto the Petri plate as a thin film and dried at 40 ☌ for 24 h. PVA was dissolved in double distilled water and collagen solution was prepared by acetic acid (A.R. (20) The PVA acts as a base matrix for the preparation of PVA/collagen blend films doped with different concentrations of B1 by the solution casting method. The protein patterns of the isolated collagen sample was analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis according to the method of Laemmli using 10% resolving gel and 6% stacking gel. The isolated collagen was characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and UV analysis. Amicon Ultra centrifugal filters (Millipore) 100 kDa cutoff filters were used for concentrating the proteins. longiceps) was taken for the study using the method reported by Nagai and Suzuki (2000a) with a little modification. The isolated ASC from the skin of Indian oil sardine ( S. Therefore, the study aimed to prepare PVA/collagen/ B1 blend films where the acid-soluble collagen (ASC) was isolated from the skin of Indian oil sardine ( Sardinella longiceps). The present study investigates the development of distinct UV-A and UV-B radiation filtering materials through the introduction of a heterocyclic bischalcone derivative to study the UV enduring property.
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